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Fig. 2 | Reproductive Biology and Endocrinology

Fig. 2

From: The involvement of osteopontin and matrix metalloproteinase- 9 in the migration of endometrial epithelial cells in patients with endometriosis

Fig. 2

E2 induced OPN and MMP-9 expression in EECs. A dose–dependent response (a) and a time-dependent response (b) based on real-time RT-PCR depicts the changes in OPN and MMP-9 expression after stage III/IV EEECs were treated with E2. Typical bands of western blots and the histogram are shown for E2 treatment at the optimum concentration (10 nM) and time point (48 h) grouped by rAFS staging (c) or by menstrual cycle (d). (e) The detection of MMP-9 cell-surface expression levels by flow cytometry. p, proliferative phase; s, secretory phase. 1, 3, 5 and 7 are the untreated condition; 2, 4, 6 and 8 are the E2 treatment. The histogram represents three independent assays. Data are presented as the mean ± S.E. *, p < 0.05 for differences between cell types with the same treatment

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