Table 2 Oligonucleotide primer sequences
Genes | Product size | Forward | Reverse |
|---|---|---|---|
β-actin | 188 bp | 5’-ACGGAACCACAGTTTATCATC-3’ | 5’-GTCCCAGTCTTCAACTATACC-3’ |
RPL-15 | 194 bp | 5’-TGTGATGCGTTTCCTCCTTGG-3’ | 5’-CCATAGGTTGCACCTTTTGGG-3’ |
EF1-α | 90 bp | 5’-AGCAGACTTTGTGACCTTGCC-3’ | 5’-TGACATGAGACAGACGGTTGC-3’ |
Visfatin | 502 bp | 5’-CGTTCAGCCCATTTGGTGA-3’ | 5’-AGTGGTGCCTCTGGACTTCG-3’ |
Chemerin | 314 bp | 5’-CGCGTGGTGAAGGATGTG-3’ | 5’-CGACTGCTCCCTAAAGAGGAACT-3’ |
CMKLR1 | 403 bp | 5’-CGGTCAACGCCATTTGGT-3’ | 5’-GGGTAGGAAGATGTTGAAGAGGAA-3’ |
CCRL2 | 391 bp | 5’-CACGCAGTGTTTGCTTTAAAAGC-3’ | 5’-CAACAGCCCACGTGACAATG-3’ |
GPR1 | 250 bp | 5’-ACCTGCCTGAGGAAGAAGAA-3’ | 5’-AAAGGCCAGTGGAAGCCCAT--3’ |
Adiponectin | 350 bp | 5’-ACAGGTGCAGAAGGACCGAG-3’ | 5’-AAGACAGAGCCGCTTGCTTG-3’ |
AdipoR1 | 350 bp | 5’-GAATACACACCGAGACGGGC-3’ | 5’-GCCCAAGACGCAGACAATGG-3’ |
AdipoR2 | 345 bp | 5’-GAGACTGGCAACATCTGGAC-3’ | 5’-TGCGATGCCCAGGACACAAA-3’ |