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Fig. 3 | Reproductive Biology and Endocrinology

Fig. 3

From: Expression of adiponectin, chemerin and visfatin in plasma and different tissues during a laying season in turkeys

Fig. 3

ADP (a), Visf (b), and Chem (c), mRNA expression in theca and granulosa cells from F3/4 and F1 hierarchical follicles. Relative expression of ADP (a), Visf (b), and Chem (c), mRNA in theca and granulosa cells from turkey F3/4 and F1 hierarchical follicles, based on real-time RT-PCR. Total RNA from all types of ovarian cells and hierarchical follicles was treated with DNAse and reverse transcribed as described in Materials and Methods. cDNAs were used in separate real-time quantitative PCR analyses of ADP, Visf and Chem mRNA or reference genes with SYBR green as the dye. ADP, Visf and Chem expressions were measured relative to the geometric mean of the expression of three reference genes as described in Materials and Methods. Results are means ± SEM from six different animals in each follicular stage. Different capital letters indicate a significant effect of the type of cells (Theca vs Granulosa cells) whereas lower case letters indicate a significant effect of the follicular stage (F3/4 vs F1)

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