Fig. 2

KLF12 overexpression affected decidualization in a mouse model of artificial decidualization. a Experimental scheme. Mice were ovariectomized, treated with adenovirus, subjected to an E2/P4 regimen to elicit the estrous cycle, and injected with oil as a decidualization stimulus. The uteri were collected at 5 days after stimulation. b Representative uteri after artificial induction of decidualization in the mice. S and US denote the stimulated and unstimulated uterine horns, respectively. c The fold changes in the uterine weights of stimulated horns compared with those of unstimulated horns indicates the extent of decidualization in female mice injected with control Ad-LacZ (n = 6) or Ad-Flag-KLF12 (n = 6) on day 5 after stimulation. **P < 0.01. d After artificial induction of decidualization, mouse uterine sections were stained with hematoxylin and eosin. myo, myometrium; s, stroma; le, luminal epithelium; and ge, glandular epithelium. Bar = 500 μm. e mRNA expression level of dPRP during in vivo decidualization was measured by real-time PCR in the Ad-Flag-KLF12-treated mice (n = 6). *P < 0.05 compared with the Ad-LacZ-treated mice (n = 6). f The FOXO1 mRNA level was measured by real-time PCR in the Ad-Flag-KLF12-treated (n = 18) and Ad-LacZ-treated mice (n = 14) after 5 days of oil infusion. *P < 0.05