Ampulla:
|
Microarray
|
Real-time RT-PCR
|
Gene Symbol
|
FP vs. LP
|
FP vs. LP
|
FP Mean
|
SEM
|
LP Mean
|
SEM
|
Fold-Change
|
P-value
|
Fold-Change
|
P-value
|
NTS
|
32.44
|
<0.001
|
1.05
|
0.12
|
0.007
|
0.001
|
150
|
<0.001
|
BSP3
|
−1.09
|
0.794
|
ND
| | | | | |
LDHA
|
2.22
|
<0.001
|
1.13
|
0.20
|
0.43
|
0.10
|
2.63
|
0.002
|
CCND2
|
1.23
|
0.352
|
1.19
|
0.21
|
0.91
|
0.12
|
1.31
|
0.273
|
EGR1
|
−9.13
|
0.005
|
1.19
|
0.23
|
111.9
|
34.2
|
−94
|
<.001
|
HSD17B7
|
1.03
|
0.762
|
1.07
|
0.21
|
0.86
|
0.12
|
1.24
|
0.361
|
Isthmus:
|
Microarray
|
Real-time RT-PCR
|
Gene Symbol
|
FP vs. LP
|
FP vs. LP
|
FP Mean
|
SEM
|
LP Mean
|
SEM
|
Fold-Change
|
P-value
|
Fold-Change
|
P-value
|
NTS
|
21.57
|
<0.001
|
1.51
|
0.47
|
0.017
|
0.008
|
88
|
<0.001
|
BSP3
|
−7.57561
|
<0.001
|
1.91
|
0.74
|
30.47
|
11.39
|
−16
|
0.013
|
LDHA
|
2.33775
|
<0.001
|
1.05
|
0.12
|
0.29
|
0.08
|
3.64
|
<0.001
|
CCND2
|
2.36116
|
0.003
|
1.09
|
0.18
|
0.80
|
0.17
|
1.38
|
0.238
|
EGR1
|
5.35334
|
0.019
|
1.07
|
0.13
|
108.4
|
35.2
|
−101
|
<0.001
|
HSD17B7
|
−1.13469
|
0.271
|
1.05
|
0.12
|
1.76
|
0.36
|
−1.67
|
0.251
|
- For statistical analysis of the microarray dataset, an F-test on least-square means was used to determine significance of each transcript in each comparison. Benjamini-hochberg multiple testing correction for false discovery rate (FDR) was applied and significance set to FDR adjusted P-value < 0.05. A post-hoc pairwise comparison of FP compared to LP epithelial cells was completed using Fisher’s Least Significant Difference (LSD) to determine which means differed
- NTS: neurotensin, BSP3: binder of sperm 3, LDHA: lactate dehydrogenase A, CCND2: cyclin D2, EGR1: early growth response 1, HSD17B7: hydroxysteroid (17β) dehydrogenase 7
- For the analysis of gene expression by real-time RT-PCR, mean level of expression and SEM are indicated for FP and LP epithelial cells of the ampulla and isthmus, as well as fold change in relative expression (FP vs. LP). For statistical analysis of relative gene expression by real-time RT-PCR, P-values to determine the significance of each transcript were determined using the Student’s t-test
- ND: Not detectable by real-time RT-PCR