Fig. 3
From: A functional variant in the UBE2B gene promoter is associated with idiopathic azoospermia
Transactivation of WT and three UBE2B mutant promoters by SP1. The pCMV-SP1 and the WT or mutant forms of the UBE2B promoter that were cotransfected into HeLa, 293FT and TM4 cells. UBE2B promoter activity was analyzed using luciferase assay. Compared with WT and other mutants, SP1 failed to activate the UBE2B Chr5.133706925 A > G promoter. The NC: pCMV empty vector was cotransfected with the WT UBE2B promoter. Fold induction is shown as the ratio of WT or mutants to the average of NC. (* P < 0.01)