Fig. 5

Expression of cytokeratin-7 and β-hCG in isolated trophoblast cells after 72 h in culture. a Primary trophoblast cells isolated from human term placentas were culture for 72 h and stained with cytokeratin-7 (green). Nuclei were stained with DAPI (blue). b Primary trophoblast cells isolated from human term placentas were culture for 72 h and stained with β-hCG (red). Nuclei were stained with DAPI (blue). c Merge of cytokeratin-7 and β-hCG immunostaining images. Confocal microscopy, scale bar: 50 μm