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Table 1 List of primers and TaqMan Probes used for the semi-quantitative RT-PCR

From: In vitro decidualisation of canine uterine stromal cells

Primer Accession numbers Primer sequence Product length (bp)
GAPDH AB028142 Forward: 5′-GCT GCC AAA TAT GAC GAC ATC A-3′ 75
Reverse: 5′-GTA GCC CAG GAT GCC TTT GAG-3′
TaqMan Probe: 5′-TCC CTC CGA TGC CTG CTT CAC TAC CTT-3′
18SrRNA FJ797658 Forward: 5′-GTC GCT CGC TCC TCT CCT ACT-3′ 125
Reverse: 5′-GGC TGA CCG GGT TGG TTT-3′
TaqMan Probe: 5′-ACA TGC CGA CGG GCG CTG AC-3′
IGFR1 XM545828 Forward: 5′-GGA CGT GCC TGG CAT-3′ 119
Reverse: 5′-CAC TCT TAG CCC CAC GGA TGT-3′
TaqMan Probe: 5′-AGC CCT GGA CGC AGT ATG CGG-3′
PRLR HQ267784 Forward: 5′-GGA TCT TTG CCG TTC TTT-3′ 92
Reverse: 5′-AAG GAT GCA GGT CAC CAT GCT AT-3′
TaqMan Probe: 5′-ATT ATG GTC GTA GCA GTG GCT TTG AAA GGC-3′
PRL NM_001008275 Forward: 5′-CAAGCC CAA CAG ATC CAC CAT-3′ 104
Reverse: 5′-ATC CCC CGC ACT TCT GTG A-3′
TaqMan Probe: 5′-CTG AGG GTG CTG CGC TCC TGG-3′
PGR NM_001003074 Forward: 5′-CGA GTC ATT ACC TCA GAA GAT TTG TTT-3′ 113
Reverse: 5′-CTT CCA TTG CCC TTT TAA AGA AGA-3′
TaqMan Probe: 5′-AAG CAT CAG GCT GTC ATT ATG GTG TCC TAA CTT-3′
ESR1 (ERα) XM533454 Forward: 5′-CCC ATG GAG GAG ACA AAC CA-3′ 93
Reverse: 5′-CCC TGC CTC GGT GAT ATA-3′
TaqMan Probe: 5′-CAC GGG CCC AAC TTC ATC ACA TTC C-3′
ESR2 (ERβ) XM861041 Forward: 5′-CCC AGC CCC TTC A-3′ 78
Reverse: 5′-AAT CAT ATG CAC GAG TTC CTT GTC-3′
TaqMan Probe: 5′-CCT CCA TGA TGA TGT CCC TGA CC-3′
PTGS2 (COX2) HQ110882 Forward: 5′-GGA GCA TAA CAG AGT GTG TGA TGT G-3′ 87
Reverse: 5′-AAG TAT TAG CCT GCT CGT CTG GAA T-3′
TaqMan Probe: 5′-CGC TCA TCA TCC CAT TCT GGG TGC-3′
AKR1C3 (PGFS) NM_001012344 Forward: 5′-AGG GCT TGC CAA GTC TAT TGG-3′ 74
Reverse: 5′-GCC TTG GCT TGC TCA GGA T-3′
TaqMan Probe: 5′-TCC AAC TTT AAC CGC AGG CAG CTG G-3′
PTGES NM_001122854 Forward: 5′-GTC CTG GCG CTG GTG AGT-3′ 89
Reverse: 5′-ATG ACA GCC ACC ACG TAC ATC-3′
TaqMan Probe: 5′-TCC CAG CCT TCC TGC TCT GCA GC-3′
PTGFR (FP) NM_001048097 Forward: 5′-ACC AGT CGA ACA TCC TTT GCA-3′ 86
Reverse: 5′-GGC CAT CAC ACT GCC TAG AAA-3′
TaqMan Probe: 5′-CAT GGT GTT CTC CGG TCT GTG CCC-3′
PTGER2 (EP2) AF075602 Forward: 5′-CAC CCT GCT GCT GCT TCT C-3′ 78
Reverse: 5′-CGG TGC ATG CGG ATG AG-3′
TaqMan Probe: 5′-TGC TCG CCT GCA ACT TTC AGC GTC-3′
PTGER4 (EP4) NM_001003054 Forward: 5′-AAA TCA GCA AAA ACC CAG ACT TG-3′ 96
Reverse: 5′-GCA CGG TCT TCC GCA GAA-3′
TaqMan Probe: 5′-ATC CGA ATT GCT GCT GTG AAC CCT ATC C-3′
SLCO2A1 (PGT) NM_001011558 Forward: 5′-TGC AGC ACT AGG AAT GCT GTT C-3′ 116
Reverse: 5′-GGG CGC AGA GAA TCA TGG A-3′
TaqMan Probe: 5′-TCT GCA AAC CAT TCC CCG CGT G-3′