Fig. 8
From: In vitro decidualisation of canine uterine stromal cells
Expression of cyclooxygenase 2 (COX2, PTGS2) and PGE2-synthase (PGES, PTGES) as determined by Western blot analysis. Following stimulation, cells were collected and homogenized and 20 μg of the lysate was used for Western blots. The averaged standardized optical density (SOD) is shown. GAPDH was used for loading control. The cell culture experiments were performed independently at least three times using cells isolated from different animals. Representative Western blots are shown. Student’s t-test was applied to test the effect of treatment on protein expression: in a (*) indicates P < 0.01, (**) indicates P < 0.004, (***) indicates P < 0.0001; in b (*) indicates P < 0.008