Fig. 7
From: In vitro decidualisation of canine uterine stromal cells
Expression of cyclooxygenase 2 (COX2, PTGS2), PGE2-synthase (PGES, PTGES), PGE2 receptors: EP4 (PTGER4) and EP2 (PTGER2), PGF2α-synthase (PGFS/AKR1C3), PGF2α receptor (PTGFR, FP) and PG-transporter (PGT, SLCO2A1), as determined by Real Time (TaqMan) PCR. Canine primary stromal cells were cultured for 24, 48 and 72 h in the presence of increasing dbcAMP concentrations. One-way ANOVA was applied (24 h in COX2 P < 0.005, 48 h in COX2 P < 0.01, 72 h in COX2 P < 0.0001; 48 h in PGES P < 0.01, 72 h in PGES < 0.03; 24 h in EP2 P < 0.0001, 72 h in PGFS P < 0.03, 48 h in FP P < 0.03, 72 h in FP P < 0.01), followed by the Tukey-Kramer Multiple Comparison Test; all samples were compared against the non-treated control in each group. Different letters indicate P < 0.05. Numerical data are presented as the mean ± standard deviation (SD)