Fig. 3
Effect of trans-10, cis-12 conjugated linoleic acid (CLA) on oocyte membrane permeability to water and cryoprotectants. a Time course of the oocyte volume change after the addition of sucrose (200 mM) to the extracellular media (oocyte shrinkage). The rate at which the oocyte shrinks is visibly slower when oocytes are matured in the presence of CLA. b Time course of the oocyte volume change after the addition of 10 % EG and 10 % DMSO to the extracellular media. The first water outflow (shrinkage) is followed by an influx of cryoprotectant with consequent oocyte reswelling. The rate of cryoprotectant influx is slower for oocytes matured in the presence of CLA. c Oocyte membrane permeability (%, relative to control) for oocytes control and oocytes matured with CLA. Pf, permeability to water. Ps, permeability to cryoprotectants (10 % EG and 10 % DMSO). *** indicates significant difference (p < 0.001)