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Fig. 4 | Reproductive Biology and Endocrinology

Fig. 4

From: Functional characterization of the domains of the bovine binder of SPerm 5 (BSP5) protein

Fig. 4

Effect of recombinant BSP5 constructs on the viability of epididymal sperm incubated with heparin. Sperm collected from epididymis were incubated for 20 min alone or in media containing 40 μg/ml of constructs N, A and B, 60 μg/ml of constructs NA, AB and FL, 60 μg/ml Trx-His-S or 30 μg/ml native BSP5. Sperm were then washed, incubated with 12 μg/ml of heparin for 5 h. Viability was assessed by staining with eosin/nigrosin solutions. Data are presented as the mean ± SD of three independent experiments

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