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Fig. 3 | Reproductive Biology and Endocrinology

Fig. 3

From: Functional characterization of the domains of the bovine binder of SPerm 5 (BSP5) protein

Fig. 3

Effect of recombinant BSP5 constructs on lyso-PC-induced acrosome reaction of epididymal sperm incubated with heparin. Sperm collected from cauda epididymis were incubated for 20 min alone or in media containing increasing concentrations of the different BSP5 constructs, 60 μg/ml Trx-His-S or 30 μg/ml native BSP5. Sperm were then washed, incubated with 12 μg/ml of heparin for 5 h and incubated an additional 15 min in presence (white bars) or absence (black bars) of 100 μg/ml lyso-PC. Acrosome reaction was assessed by naphtol yellow S-erythrosin B staining. Data are presented as the mean ± SD of three independent experiments. Differences compared to control (no proteins added) were analyzed by one-way covariance analysis followed by LSD multiple comparison tests (* p < 0.05). Panels (a-f) correspond respectively to Domain N, Domain A, Domain B, Domain NA, Domain AB and Protein FL (Full length)

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