Fig. 2
From: Functional characterization of the domains of the bovine binder of SPerm 5 (BSP5) protein
SDS-PAGE pattern of purified BSP5 recombinant constructs. pET32a(+) constructs of full length (FL), A domain (A); B domain (B); NA domain (NA) and (AB) domain were expressed in E. coli origami(DE3) cells and purified by Ni2+-NTA affinity chromatography. The pET30a(+) N-terminal (N) construct was expressed in E. coli BL21(DE3) cells. Five micrograms of purified proteins were analyzed by 15Â % SDS-PAGE followed by Coomassie Blue staining