Fig. 3

Localisation of LAT transporters in term placenta. a MVM and BM expression of LAT1 and LAT2 analysed by Western blot (left) and total protein staining of the two corresponding PVDF membranes (right). Equal amounts of proteins were loaded in each lane (20 μg/lane). mwm molecular weight marker. b-c Representative immunohistochemistry images of LAT1 and LAT2 in human term placenta (N = 3 for each condition). LAT1 localisation was assessed using two different antibodies (panel b, left: antibody provided by Dr Kanai at 20 μg/ml final concentration; panel b, right: antibody by Capralogics at 40 μg/ml and 20 μg/ml final concentrations). Negative controls and Rabbit IgG staining (40 μg/ml and 20 μg/ml final concentrations) are shown. Nuclei stained with hematoxylin. MVM (black arrow), BM (black arrowhead), capillary endothelium (empty arrowhead). Scale bar 20 μm