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Fig. 1 | Reproductive Biology and Endocrinology

Fig. 1

From: Expression and functional characterisation of System L amino acid transporters in the human term placenta

Fig. 1

Decrease of System L activity after silencing of LAT1 or LAT2 transporters. PHT cells were transfected with non-targeting scramble-siRNA (si-SCR), LAT1-siRNA (si-LAT1) or LAT2-siRNA (si-LAT2). a mRNA expression of LAT1 and LAT2, normalized against the housekeeping genes SDHA and TBP. b Representative immunoblots of LAT1, LAT2 and the corresponding beta-actin following si-RNA treatments. Histograms illustrate relative protein expression. c System L amino acid transport activity following si-RNA treatments. Mean of controls was assigned an arbitrary value of 1 and data represents fold change from si-SCR; mean + SEM, N = 3. Student t-test (a and b) or one-way ANOVA (c), *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 vs control (si-SCR)

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