Fig. 6

Down-regulation of NDRG2 expression in ESCs inhibits in vitro decidualization. Cultured mouse ESCs were transfected with NDRG2-targeting siRNAs (100 nM) (a non-targeting siRNA was used as a control) at the time of plating. Quantitative PCR analyses of NDRG2 mRNA expression (a) and DTPRP mRNA expression (b) in ESCs transfected with NDRG2-targeting siRNAs or non-targeting siRNAs at 24 h and 48 h. Western blot (c) and densitometric analyses (d) of NDRG2 protein levels in ESCs at 72 h after transfection. The relative fold induction of NDRG2 protein expression compared with its expression in non-siRNA-treated group is shown. The values represent the mean ± SEM, as determined from three separate experiments. *, significantly different (P < 0.05)