Fig. 5
In vitro decidualization of mouse primary ESCs. ESCs isolated from the uteri of day 4 pregnant mice were cultured in the presence of P4 and E2. a Immunocytochemical detection of vimentin and cytokeratin in cultured ESCs. b Quantitative PCR analysis of DTPRP mRNA expression in ESCs cultured for up to 72 h. c Western blot analysis of NDRG2 protein expression in ESCs cultured for up to 72 h. Densitometric analyses of NDRG2 at each time point compared with 0 h is shown. d Quantitative PCR analysis of NDRG2 mRNA expression in cultured ESCs. The relative fold induction of NDRG2 mRNA expression at each time point compared with its expression in the 0 h sample is shown. The values represent the mean ± SEM, as determined from three separate experiments. *, significantly different (P < 0.05)