Fig. 1
Effects of MEHP with and without indomethacin (INDO) or NS-398 on prostaglandin release from primary placental macrophage cells or THP-1 cells. a Placental macrophages were treated for 2, 4, 8, or 24 h with DMSO (0.05 % v/v; solvent control) or 180 μM MEHP. b and c Placental macrophages were treated for 24 h with 0 (0.05 % v/v DMSO; solvent control), 10, 45, 90, or 180 μM MEHP. d and e Placental macrophages were treated for 24 h with DMSO (0.05 % v/v; solvent control) or 180 μM MEHP with or without 1 μM indomethacin or 1 μM NS-398. f and g THP-1 cells were treated for 24 h with DMSO (0.05 % v/v; solvent control) or 180 μM MEHP with or without 10 μM indomethacin or 1 μM NS-398. EIAs for PGE2 or PGF2α were performed on cell culture medium as described in the “Methods” section. N = 3-4 women per placental macrophage experiment, 1–3 replicates within each treatment group per experiment. N = 3 experiments for THP-1 cells. *p < 0.05, **p < 0.01, compared to solvent controls