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Fig. 4 | Reproductive Biology and Endocrinology

Fig. 4

From: The effect of lysophosphatidic acid during in vitro maturation of bovine cumulus–oocyte complexes: cumulus expansion, glucose metabolism and expression of genes involved in the ovulatory cascade, oocyte and blastocyst competence

Fig. 4

The effect of LPA (10−5M) supplementation of oocyte maturation medium on apoptosis in COCs. Panel a depicts representative fluorescent images of bovine COCs used to TUNEL labeling: a negative control, b positive control, c, d control COCs, e, f COCs matured in the presence of LPA. White arrows indicate TUNEL stained apoptotic nuclei (green) in contrast to DAPI stained nuclei (blue). Bars = 100 μm. Panel b depicts quantitative analysis of LPA effect on apoptosis in COCs. The data are presented as a percentage of TUNEL positive apoptotic cells within all detected DAPI positive cells and expressed as mean ± SEM. Different letters indicate significant differences (P <0.05), as determined by Student’s t-test

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