Fig. 4

Representative RXFP1 and RXFP2 western immunoblotting gels. Equal amounts (20–60 μg) of total proteins obtained from various pig tissues were resolved on 4–12.5 % SDS-PAGE gels, transferred to PVDF membranes, and immunoblotted with commercial anti-RXFP1 (sc-50328) and anti-RXFP2 (sc-50327) antibodies. Proteins were extracted from prostate (PR), seminal vesicles (SV), vas deferens (VD), and epididymis sections of cauda (CA), corpus (CO), caput (CP), and testicular homogenate (TE). Total protein extracts of sow ovarian corpus lutea (CL) were used as the positive control. Immunodetected proteins were revealed using a colorimetric technique (Novex® HRP Chromogenic). MW1 and MW2 correspond to two different molecular markers, MagicMark™ XP Western Protein Standard (1 = 220 kDa; 2 = 120 kDa; 3 = 100 kDa; 4 = 80 kDa; 5 = 60 kDa; 6 = 50 kDa; 7 = 40 kDa; 8 = 30 kDa; 9 = 20 kDa