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Table 1 Experimental design

From: The periovulatory endocrine milieu affects the uterine redox environment in beef cows

Procedure

Daytime

SF–SCL

LF–LCL

First PGF of pre-synchronization

D–34

D–34

Second PGF of pre-synchronization

D–20

D–20

P4 device insertion + EB injection

D–10

D–10

PGF*

NO**

D–10

P4 device withdrawal + PGF***

D–1.25 to D–1.5

D–1.75 to D–2.5

GnRH injection

D0

D0

Blood sampling

D0, D2, D6 and D7

D0, D2, D6 and D7

Slaughter

D7

D7

  1. Abbreviations: PGF prostaglandin F2α (sodium cloprostenol; Sincrocio®, Ourofino Saúde Animal, Cravinhos, Brazil), P4 device progesterone-releasing device (Sincrogest; Ourofino Saúde Animal, Cravinhos, SP, Brazil), EB Estradiol benzoate (Sincrodiol®, Ourofino Saúde Animal), GnRH gonadotropin-releasing hormone (Sincroforte®, Ourofino Saúde Animal). *PGF given on the beginning of protocol; **Cows of SF-SCL did not receive PGF injection on D–10; ***PGF given on the end of protocol.