Skip to main content
Figure 1 | Reproductive Biology and Endocrinology

Figure 1

From: The periovulatory endocrine milieu affects the uterine redox environment in beef cows

Figure 1

Synchronization protocol. Cows were pre-synchronized via two injections of prostaglandin F2α (PGF) administered 14 days apart, starting on protocol Day −34 (D–34). On D–10, the cows received a progesterone-releasing device (P4 device) (Sincrogest; Ourofino Saúde Animal, Cravinhos, SP, Brazil) and an injection of 2 mg of estradiol benzoate (EB; Sincrodiol; Ourofino Saúde Animal). Also, on D–10, the cows were prearranged between large follicle-large corpus luteum group (LF-LCL) or small follicle-small corpus luteum group (SF-SCL) and only cows of LF-LCL received an injection of PGF (0.5 mg of sodium cloprostenol; Sincrocio; Ourofino Saúde Animal). Between D–1.75 and D–2.5 the P4 device was removed from cows of LF-LCL. On the cows of SF-SCL the P4 device was removed between D–1.25 and D–1.5. All the animals received an injection of PGF in the same time of P4 device withdrawal and a second PGF injection 6 h later. On D0, in both groups, the ovulation was induced with an injection of gonadotropin-releasing hormone (GnRH; 0.01 mg of buserelin acetate; Sincroforte; Ourofino Saúde Animal). Blood sampling was conducted on D0, D2, D6 and D7. The cows were slaughtered on D7, which was the end point for endometrial tissue collection.

Back to article page