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Figure 3 | Reproductive Biology and Endocrinology

Figure 3

From: Activity of the porcine gonadotropin-releasing hormone receptor gene promoter is partially conferred by a distal gonadotrope specific element (GSE) within an upstream enhancing region, two proximal GSEs and a retinoid X receptor binding site

Figure 3

The RXR binding site important for GnRHR promoter activity (-279/-274) binds RXRα and β. A, EMSAs performed with nuclear extracts and a probe for the RXR region of the promoter (specific complex indicated by arrow). Nuclear extracts were also incubated with antibodies directed against the RXR subtypes (α, β and γ) or rabbit IgG before the addition of probe (supershift indicated by arrow). B, Cells were transiently transfected with -5118pGL3, μRXRpGL3 or pGL3. An asterisk indicates values that are greater than pGL3 (P < 0.05).

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Reproductive Biology and Endocrinology

ISSN: 1477-7827

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