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Figure 2 | Reproductive Biology and Endocrinology

Figure 2

From: Activity of the porcine gonadotropin-releasing hormone receptor gene promoter is partially conferred by a distal gonadotrope specific element (GSE) within an upstream enhancing region, two proximal GSEs and a retinoid X receptor binding site

Figure 2

Proximal GSEs located at -179/-171 and -315/-310 of the porcine GnRHR promoter bind SF1. A, EMSAs performed with nuclear extracts and probes for the regions spanning either SF1 I or SF1 II. In addition, nuclear extracts were incubated with antiserum directed against SF1 (including its DNA binding domain) or normal rabbit IgG before the addition of radiolabeled probe (specific complex abrogation indicated by arrow). Electrophoresis of the SF1 I EMSA (left) was performed for an extended time, thus, free probe was run off the gel. B, Cells were transfected with -5118pGL3, μSF1IpGL3, μSF1IIpGL3 or pGL3. An asterisk indicates values that are greater than pGL3 (P < 0.05).

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Reproductive Biology and Endocrinology

ISSN: 1477-7827

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