Figure 2
From: Comparative proteomic analysis of spermatozoa isolated by swim-up or density gradient centrifugation
Location of spots differing ≥1.5-fold in their intensity on comparison of DC with SU cells. Sypro Ruby-stained gels were stained with colloidal Coomassie Blue for quantitative analysis. A representative protein map shows encircled in red the 11 spots for which significant differences ≥1.5-fold (p < 0.05, Anova) were found between DC and SU group. The differences are either DC > SU or SU > DC, as indicated in Figure 3 and Table 1. The 6 spots that are numbered were considered to be reliable because located in areas of the gels not affected by horizontal streaking and/or a strong background.