Figure 3

The phosphorylation of pAMPK. The phosphorylation of pAMPK in KGN cells treated with AICAR (1 mM) or metformin (1 mM) for 5 min to 24 h (n = 4). GAPDH is shown as the internal control. A: Representative blots illustrating the effect of treatment on pAMPK and GAPDH by AICAR for 5 min to 24 h. C: Representative blots illustrating the effect of treatment on pAMPK and GAPDH by metformin for 5 min to 24 h. The blot layout is in the same order as the graph in panels B and D, respectively.