Figure 4
From: Beneficial effects of relaxin on motility characteristics of stored boar spermatozoa
Fluorescence microscopy evaluation of sperm viability after storage. Representative images show spermatozoa stained for mitochondria membrane potential (JC-1; Upper panel) or plasma membrane integrity (PI; Lower panel) evaluation. Upon excitation at 488 nm, spermatozoa with intact (high potential) or damaged (low potential) mitochondrial membranes fluoresce red-orange or green, respectively, while those with damaged plasma membrane fluoresce red. Magnification 200X.