miR-181a inhibits KLF12 expression. (A) Putative miR-181a-targeting sites in the human and mouse KLF12 mRNA 3′UTRs. (B) Analysis of miR-181a modulation on wild-type or mutant KLF12 3′UTR luciferase reporter plasmids. **p < 0.01 (n = 3). hESC were infected with Ad-miR-181a or transfected with miR-181a inhibitor as indicated for 48 h. qRT-PCR and western blotting were performed to examine endogenous KLF12 mRNA (C) and protein (D) expression. *p < 0.05, compared with control group, **p < 0.01, compared with the Ad-LacZ group.