Figure 1

miR-181a induces hESC decidualization in vitro . (A) Expression pattern of miR-181a in hESC treated with 0.5 mM 8-Br-cAMP and 1 μM MPA (8Br + MPA) for different periods of time (3, 6, 12, 24, and 48 h, respectively) was evaluated by qRT-PCR. *p < 0.05, **p < 0.01. hESC were infected with Ad-miR-181a or Ad-LacZ (MOI = 100). After 24 h, these cells were treated with 0.5 mM 8-Br-cAMP and 1 μM MPA as indicated for an additional 72 h. miR-181a (B), FOXO1A (C), PRL (D), IGFBP1 (E), DCN (F), and TIMP3 (G) mRNA levels were measured by qRT-PCR. **p < 0.01, bars labeled with different letters indicate statistically significant differences (p < 0.05). (H) hESC were infected with Ad-miR-181a or Ad-LacZ (MOI = 100) for 24 h followed by treatment with 0.5 mM 8-Br-cAMP and 1 μM MPA for the indicated times. Prolactin released into the medium was detected by ELFA. *p < 0.05, **p < 0.01, compared with Ad-LacZ treated with 8-Br-cAMP and MPA. (I) Immunofluorescence using Alexa Fluor 594-conjugated phalloidin to label actin filaments was performed to analyze the morphological transformation of hESC.