Figure 6

Effects of slow freezing and vitrification on mitochondrial activity, intracellular ROS levels and mt/ROS colocalization in single mouse morulae and blastocysts, as related to mt pattern. In each group, energy status (Panel a) intracellular ROS levels (Panel b) and mt/ROS colocalization (Panel c) are expressed as in Figure 4 and Figure 5. Vitrified embryos with P/P pattern had significantly higher energy status than their SA counterparts and significantly higher ROS levels and mt/ROS colocalization than P/P controls (P < 0.05). Numbers of analyzed embryos per group are indicated on the bottom of each histogram. One-way ANOVA followed by Multiple Comparison Dunn’s or Dunnett’s met hods: comparisons among morula stage embryos: a,b P < 0.05; comparisons among blastocyst stage embryos: c,d P < 0.05.