Figure 5

Effects of slow freezing and vitrification on mitochondrial activity, intracellular ROS levels and mt/ROS colocalization in single mouse morulae and blastocysts, as related to embryo grade. In each group, energy status (Panel a), intracellular ROS levels (Panel b) and mt/ROS colocalization (Panel c) are expressed as in Figure 4. In grade C SF morulae, energy status was significantly lower than in control grade A and in vitrified grade A morulae (P < 0.05). Numbers of analyzed embryos per group are indicated on the bottom of each histogram. One-way ANOVA followed by Multiple Comparison Dunn’s method: a,b P < 0.05.