Figure 2

Effects of slow freezing and vitrification on mitochondrial distribution pattern of mouse embryos at the morula and blastocyst stage. Percentages of embryos graded according to chromatin damage as grade A, grade B or grade C, as in Figure 1, and further divided as having perinuclear/pericortical (P/P; apple-green, blue and brick-red, respectively) or homogeneous mt distribution pattern in small aggregates (SA; pale colours). Embryos were grouped according to their developmental stage, observed after slow freezing or vitrification and compared with controls. Numbers of analyzed embryos per group are indicated on each histogram and segment. Chi square test with the Yates correction: comparisons control vs slow freezing and control vs vitrification: *P < 0.05; **P < 0.01;***P < 0.001; ****P < 0.0001; comparisons slow freezing vs vitrification: #P < 0.05; ##P < 0.01; ###P < 0.001.