Figure 2

The analysis of functional blood vessels in ovarian grafts 3 days after transplantation. A. An illustration of functional blood vessels that were observed using dextran-FITC immunostaining. B. The quantification of FITC-positive vessels in ovarian transplants from the control and VEGF₁₆₅-treated groups. In the treated mice, 8 of 15 fragments contained functional vessels compared with only 4 of 14 fragments in the control mice. C. An illustration of double staining for functional and mature blood vessels in a transplant that was treated with VEGF₁₆₅. FITC was stained brown, and α-SMA was stained red. n corresponds to the number of transplanted ovarian fragments that contained functional blood vessels. Scale bar: 200 μm. *Corresponds to a p value < 0.05. Error bar are SEM (mean standard error).