Table 3 Gene names, primers, genbank accession numbers, annealing temperature and size of amplicons
Gene | Primer sequence (5′-3’) | Genbank | Ta* | Amplicon size (bp) |
|---|---|---|---|---|
Accession No. | (°C) | |||
MEST (Peg-1) | TGGAGGCGTGCTGTCACCCA | NM_001128471 | 60 | 72 |
ACTGGGGTGAGACCCCGAGAG | 59 | |||
LEP (Leptin) | TCCAGGATGACACCAAAACCCTCA | NM_213840.1 | 58 | 95 |
GGTGACCCTCTGTTTGGAGGAGACA | 60 | |||
PPARGC1 (PGC-1α) | GGCAATTGAAGAACGTCGTGT | NM_213963.1 | 60 | 74 |
GGTCCCTCAGTTCTGTCCGT | 61 | |||
CASP1 (Caspase-1; ICE; IL1BC; P45) | GGAGACGACCCCCACCTTGC | NM_214162 | 59 | 98 |
GGAGGAACCACCGCCTGGGAT | 60 | |||
MCP1 (CCL2; SCYA2) | GGTCCTTGCCCAGCCAGATGC | NM_214214.1 | 60 | 89 |
TCATCAGCCGCTGCATCGAGA | 58 | |||
PAI-1 (SERPINE-1) | CGGACCACGGTCAAGCAGGTG | NM_213910 | 60 | 147 |
CACCAGAACCAGGCGCGTCA | 60 | |||
IL-18 | ACCAGGGACATCAAGCCGTGT | NM_213997.1 | 58 | 118 |
ACTGCCAGACCTCTAGTGAGGC | 57 | |||
ACACB (ACC2) | TGCCGTGTCCCTGTTTGGGC | NM_001206399.1 | 60 | 126 |
AGGCGCACAGCACACTGCTC | 60 | |||
FABP4 | GCAGATGACAGGAAAGTCAAGAGCA | NM_001002817.1 | 57 | 58 |
CCTGTACCAGGGCGCCTCCA | 60 | |||
HSL (LIPE) | AGTGCCTATGCTGGCGGGGA | NM_214315.1 | 60 | 116 |
CCCAGGCGGAGGTCTCGGAA | 60 | |||
LPL | GTCTCGGGCCCAGCAGCATT | NM_214286 | 59 | 150 |
GCGTGGGCTCCAAGGCTGTA | 59 | |||
CD36 | GCTGTGGCAGCTGTACCCCA | NM_001044622 | 59 | 135 |
TGGATCCGGATAGCCCCACAAT | 57 | |||
GLUT4 (SLC2A4) | CGGCATGGGTTTCCAGTATG | NM_001128433.1 | 60 | 61 |
CGCGAAGAGAAGGAAGACGTA | 58 | |||
ATGL (PNPLA2) | GGCGGAACGGCCTCCTGAAC | NM_001098605.1 | 60 | 121 |
TTGGCTCCGGCCCTCTCCTC | 60 |