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Table 2 Synthetic substrates for hESC culture

From: Human embryonic stem cell cultivation: historical perspective and evolution of xeno-free culture systems

System description/substrate

Cell type

Medium/supplements

SF 1

CD 2

XF 3

Culture period

Ref

Synthetic acrylate surfaces (PAS) with peptides: Vitronectin, bone sialo protein

hESC

X -VIVO 10

Y

N

Y

>10 passages

[89]

bFGF,TGF-β1

Activin A

Synthemax 4

hiPSC

mTeSR1

Y

N

N

10 passages

[90]

Synthemax 4

hESC

mTESR1

Y

N

N

10–20 passages

[91]

hiPSC

TESR2

Y

Y

Y

PSGro

Y

Y

Y

Nutristem XF

Y

N

Y

PMEDSAH 5 coated plates

hESC

MEF-CM6

N

N

N

25 passages

[98]

hCCM7

N

N

Y

StemPro,

Y

N

N

 

mTeSR

Y

N

N

Alkanethiol with heparin binding proteins

hESC

mTeSR1

Y

N

N

>17 passages

[92]

Rock inhibitor

PMVE-alt-MA 8

hiPSC

StemPro

Y

N

N

5 passages

[94]

APMAAm 9 hydrogels

hESC

mTeSR1

Y

N

N

20 passages

[95]

Polyethylene glycol hydrogels (PEG)

hESC

DMEM/F12 + KSR

N

N

N

9 days

[118]

bFGF

Hillex 10 microcarrier//suspension culture

hESC

KSR-XF 10

Y

N

Y

6–14 days

[123]

BRASTEM,

Y

N

Y

bFGF

   
  1. 1SF serum –free medium.
  2. 2CD chemically defined medium (excludes any media containing BSA or HSA fraction due to their unknown and variable composition).
  3. 3XF xeno-free, no animal components in medium or culture system.
  4. 4Synthemax acrylate surface coated with peptide derived from vitronectin.
  5. 5PMEDSAH Poly [2-(methacryloyloxy) ethyl dimethyl-(3-sulfopropyl) ammonium hydroxide]—.
  6. 6MEF-CM mouse embryonic fibroblast conditioned medium.
  7. 7Human cell conditioned medium.
  8. 8PMVE-alt-MA poly (methyl vinyl ether-alt-maleic anhydride.
  9. 9APMAAm aminopropylmethacrylamide—.
  10. 10Hillex polystyrene beads modified with cationic trimethyl ammonium.