Immunohistochemical localization of AQP1 and AQP5 in uterine tissue explants. Immunoperoxidase staining of AQP1 in paraffin-embedded sections of the uterine explants from pigs. Anti-AQP1 antibody labels endothelial cells of the uterine explants (arrows). Both apical and basal plasma membranes exhibited stable AQP1 labeling (A/ an example of the staining for the control and B/ from the E2-treated uterine explants for 24-h on Days 14–16 of the cycle). Immunoperoxidase labeling of AQP1 in the pig kidney cortex (Y/ positive control). The labeling is seen in both of the apical and basolateral plasma membrane in proximal tubule cells. AQP5 antibody stains epithelial cells of the uterine explants (arrows). The labeling is seen only in the apical plasma membranes of the epithelial cells (C/ an example of the staining for the control on Days 14–16 of the cycle). Continuously, 3- and 24-h treatments on Days 10–12 and 14–16 of the estrous cycle of the tissue explants with P4
J-K), FSK (L-M and
N-O) and cAMP (P-Q and
R-S), respectively, prominent AQP5 labeling is seen in both the apical and basolateral plasma membranes of the epithelial cells. A similar pattern of AQP5 labeling is also seen after 3-h treatment with AA on Days 14–16 of the estrous cycle (T). AQP5 antibody also stains smooth muscle cells (U/ an example of the staining for the control and W/ from the E2-treated uterine explants for 24-h on Days 14–16 of the cycle). The anti-AQP5 labels apical membrane of type I pulmonary epithelial cells of the pig (Z/ positive control). No staining was observed with using non-immune immunoglobulins (negative controls: X1 – AQP1 and X5 – AQP5 controls). L – lumen. Bar = 50 μm.