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Figure 4 | Reproductive Biology and Endocrinology

Figure 4

From: Progesterone, estradiol, arachidonic acid, oxytocin, forskolin and cAMP influence on aquaporin 1 and 5 expression in porcine uterine explants during the mid-luteal phase of the estrous cycle and luteolysis: an in vitro study

Figure 4

Protein content of AQP5 in uterine explants. Semi-quantitative Western blot analysis of AQP5 in homogenates of the pig uterine explants from (A) Days 10–12 (n = 5) and (B) Days 14–16 (n = 5) of the estrous cycle (each lane represents a sample from 1 pig), after treatment with progesterone (P4; 10−5 M), estradiol (E2; 10−9 M), oxytocin (OT; 10−7 M), arachidonic acid (AA; 10−5 M), forskolin (FSK; 10 μg/mL) and cyclic-AMP (cAMP; 200 μM). Different small letters (a, b, c, d) indicate significant differences (p < 0.05) between each treatment and respective control for 3- (a,b) or 24-h (c,d) incubations. Different large letters (A, B, C, D) indicate significant differences between the same treatments for 3- (A,B) or 24-h (C,D) incubations for different periods (Days 10–12 and 14–16) of the estrous cycle (p < 0.05).

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