Figure 3

Origin, meiosis I, and migration of human adult germ cells, and follicular renewal in a 28 year old women (F28). A) Dual color IHC of asymmetrically dividing OSC with CK+ (blue dashed line) OSC daughter and PS1+ (red dashed line) germ cell daughter. CK+ OSC daughter chromosomes (white arrowhead) move to the OSC end during mitotic OSC anaphase. Germ cell chromosomes (white arrows) duplicate by DNA replication (red and blue arrows) and exhibit metaphase I sister chromatid crossover (orange arrows) during meiosis I metaphase. White asterisk and dotted circle indicate PS1+ putative CD8/DR+ suicidal T cell within the germ cell (see Figures 1B, 2D,E and Ref. [36]). B) In the TA (ta) the symmetrically dividing germ cell exhibits strong nuclear (asterisks) PS1 expression, which accompanies the meiosis I telophase. Arrow indicates a germ cell moving from the TA to the upper ovarian cortex (uoc). Inset shows a detail of chromosomal crossover (orange arrows) from panel A. Red and blue arrows indicate interacting sister chromatids. C) Germ cell with a diminution of nuclear and increase of cytoplasmic PS1 staining. It begins to enter (arrowheads) the vein in the upper ovarian cortex. D) Early stage of new primary follicle formation with ZP (blue color) expression of a small oocyte captured by the CK+ (brown color) granulosa cell nest. See details in the text. Adapted from Ref. [3]: © Antonin Bukovsky.