Figure 2

Migration assay. A. Representative images obtained along the wound, at 0 and 18 hours of stimulation with BK, the B1R agonist LDBK, and of preincubation with the antagonists of the B2 and the B1 receptors, HOE-140 and AB1R respectively (× 100). B. Effect of BK on the migratory capacity of HTR-8/SVneo cells analyzed at 18 hours. Mean ± SE; *p < 0.001 when comparing BK stimulation to control, and BK stimulation with preincubation with the B2R antagonist HOE-140; incubation with the B1R agonist LDBK, with or without preincubation with its antagonist AB1R did not modify cell migration. N = 4 for each condition. C. Representative immunofluorescence of the B2R, and of Western blot of lyzed cells, both after 18 hours of incubation under control conditions and BK stimulation.