Figure 3

Western blot analysis of IL11 regulated Annexin A2 (ANXA2) protein in human endometrial epithelial cell (hEEC) membranes and in total cellular protein. The hEEC cell lines, ECC-1 and Ishikawa and primary hEEC cells were treated without (diluent control) or with IL11 (100 and/or 500 ng/ml) for 24 h and membrane proteins extracted as described in the Methods. Cell lysates were electrophoresed by SDS-PAGE and immunoblotted with ANXA2 in (A) total primary hEEC protein lysates (30 μg; left panel) and membrane primary hEEC (1 μg; right panel), (B) ECC-1 (0.1 μg) and (C) Ishikawa (0.1 μg) cells. Na⁺/K⁺-ATPase is used as a loading control for membrane proteins. Data is shown as a single photomicrograph representative of 3 independent experiments. * P ≤ 0.05; t-test.