Figure 3
Protein localization of SERPINA5, SERPINB6, SERPINF2 and SERPING1 in E 2 -active and E 2 -inactive follicles. Localization of SERPINA5 (A and B), SERPINB6 (C and D), SERPINF2 (E and F) and SERPING1 (G and H) protein was detected by immunohistochemistry. Sections (7 μm) of bovine E2-active (A, C, E and G) and E2-inactive follicles (B, D, F and H) were incubated with anti-SERPINA5, anti-SERPINB6, anti-SERPINF2 and anti-SERPING1 polyclonal antibodies. SERPINA5, SERPINB6 and SERPINF2 were detected in the GCs of E2-active and E2-inactive follicles. SERPING1 was detected in both GCs and the TL of E2-active and E2-inactive follicles. Negative control (I and J) was incubated without anti-SERPIN antibodies. Scale bars = 20 μm.