Tissue distribution of aqp1b in Japanese eel. RT-PCR was performed using 1 μg of total RNA prepared from Japanese eel tissues using the Omniscript kit (Qiagen). Amplification products were analyzed on a 2.0% agarose gel and stained by ethidium bromide. Control RT-PCR of β-actin using the same amount of cDNA is also indicated in the lower panel. Lane from the left is brain(1), eye(2), gill(3), esophagus(4), heart(5), liver(6), kidney(7), intestine(8), ovary(9), testis(10), and no template (11), respectively.