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Table 1 Comparative analytical characteristics for electrochemical, MTT and fluorescence techniques

From: Electrochemical sensors, MTT and immunofluorescence assays for monitoring the proliferation effects of cissus populnea extracts on Sertoli cells

Technique Time Reagents/Measurement Effective extract conc. (ppm) Proliferation effect LOD(cells/ml
DOX 40 min. • No additional reagents
• No background signals
• No washing needed for the cells 		10 120% 1.25 × 10(4)
MTT 4Hrs • MTT, Acidified Propanol
• Washing step required for the cells 		10 105-120% 2.5 × 10(4)
Fluorescence 20min • Syto 24 Green
• There is background effect of the solvents and medium 		10 120-150% 2.5 × 10(4)
  1. Glossary: LOD- Limit of detection; MTT-(3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide, DOX- Dissolved Oxygen Multi electrode potentiostat/microplate; Experimental conditions- Cells incubated under regular growth conditions at 37°C with 5% CO2 in air atmosphere
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