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Table 1 Comparative analytical characteristics for electrochemical, MTT and fluorescence techniques

From: Electrochemical sensors, MTT and immunofluorescence assays for monitoring the proliferation effects of cissus populnea extracts on Sertoli cells

Technique

Time

Reagents/Measurement

Effective extract conc. (ppm)

Proliferation effect

LOD(cells/ml

DOX

40 min.

• No additional reagents

• No background signals

• No washing needed for the cells

10

120%

1.25 × 10(4)

MTT

4Hrs

• MTT, Acidified Propanol

• Washing step required for the cells

10

105-120%

2.5 × 10(4)

Fluorescence

20min

• Syto 24 Green

• There is background effect of the solvents and medium

10

120-150%

2.5 × 10(4)

  1. Glossary: LOD- Limit of detection; MTT-(3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide, DOX- Dissolved Oxygen Multi electrode potentiostat/microplate; Experimental conditions- Cells incubated under regular growth conditions at 37°C with 5% CO2 in air atmosphere