Electrochemical sensors, MTT and immunofluorescence assays for monitoring the proliferation effects of cissus populnea extracts on Sertoli cells

Table 1 Comparative analytical characteristics for electrochemical, MTT and fluorescence techniques

Technique	Time	Reagents/Measurement	Effective extract conc. (ppm)	Proliferation effect	LOD(cells/ml
DOX	40 min.	• No additional reagents • No background signals • No washing needed for the cells	10	120%	1.25 × 10(4)
MTT	4Hrs	• MTT, Acidified Propanol • Washing step required for the cells	10	105-120%	2.5 × 10(4)
Fluorescence	20min	• Syto 24 Green • There is background effect of the solvents and medium	10	120-150%	2.5 × 10(4)

Glossary: LOD- Limit of detection; MTT-(3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide, DOX- Dissolved Oxygen Multi electrode potentiostat/microplate; Experimental conditions- Cells incubated under regular growth conditions at 37°C with 5% CO₂ in air atmosphere