Figure 3From: Electrochemical sensors, MTT and immunofluorescence assays for monitoring the proliferation effects of cissus populnea extracts on Sertoli cellsMTT results showing the residual activity taken at normal incubation conditions for 4 hrs in air atmosphere at concentration of 10 ppm intervals. (a) Crude extracts (b) hexane fraction (c) ethyl acetate fraction (d) butanol fraction. The absorbance was taken at 570 nm, 200 uL of acidified 2-propanol with 0.04 N HCl was added to each well and mixed in order to dissolve the precipitate and ensure color development.Back to article page