Figure 2From: Electrochemical sensors, MTT and immunofluorescence assays for monitoring the proliferation effects of cissus populnea extracts on Sertoli cellsUV/Vis spectroscopic cell optimization for MTT and fluorescence technique. Absorbance was measured at 527 nm for MTT and 485 nm for fluorescence. Optimum cell culture conditions: 5-10% CO2, 90% humidity and a constant temperature of 37°C.Back to article page