Figure 6
The microvesicles from placenta explants contain both full-length and truncated LHCGR isoforms as well as selectively enriched proteins. a), the placenta tissue extracts (Placenta) as well as proteins extracted from placenta explant microvesicles (PES) released into the culture medium at 24h (PES-24h) and 48h (PES-48h) were separated in 4-12% polyacrylamide SDS gels. The selective loss of proteins of Mr 50K-20K in the microvesicle fraction is indicated by a bracket; b), microvesicles purified by ultracentrifugation of the culture medium were examined by transmission electron microscopy; c) and d), microvesicle proteins include LHCGR as shown by c), Western blot probed with LHR29 monoclonal antibody and subsequently d), stained with coomassie blue.