Figure 5

Selective secretion of proteins from transfected cells through microvesicles. a), the whole cell extracts (lane 2, Cont), the culture supernatants (D1 and D4) following transfection with LHCGR 291 cDNA were centrifuged at low and high speed to remove the cell debris and subsequently concentrated prior to electrophoresis in 4-12% Bis-Tris polyacrylamide gels; b), same as a) except the medium concentrated from LHCGR 291 cDNA transfection from D1-D7 are shown; c), shows that proteins secreted from the mock transfected as well as cDNA transfected cells are similar, the dark and light arrows indicate quantitative enrichment and reduction of proteins secreted in the medium respectively; d), electron micrographs of D4 culture supernatents concentrated by ultracentrifugation (left panel) and by ultrafiltration (right panel). The microvesicle shown in the inset is a 12-fold magnification of that seen at the right-bottom corner of the figure, below the inset.