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Table 1 PIF secretion by mouse embryos at day 0 and day 3 of culture

From: PreImplantation Factor (PIF) correlates with early mammalian embryo development-bovine and murine models

Day 0

Day 3

3.2

26.2

56.4

35.4

23.1

83.9

9.5

33.6

 

34.5

 

50.1

 

62.6

Mean +/-SEM 23.7+/-13.5

46.6+/-7.7

  1. Mouse embryos were cultured at 2-cell stage for 4 hours (N = 102-110/embryos) and the media was collected for analysis from 4 different culture media and analyzed for PIF levels. Also, mouse embryos were cultured at 2-cell stage (N = 10-15/embryos) for 72 hours and the media was collected for PIF analysis from 7 different cultures. Results are mean values+/- SEM of results of independent cultures. Chemiluminescent ELISA. Background levels were substracted from each experiment. Data is expressed as ng/ml.