Day 0 | Day 3 |
---|
3.2 | 26.2 |
56.4 | 35.4 |
23.1 | 83.9 |
9.5 | 33.6 |
 | 34.5 |
 | 50.1 |
 | 62.6 |
Mean +/-SEM 23.7+/-13.5 | 46.6+/-7.7 |
- Mouse embryos were cultured at 2-cell stage for 4 hours (N = 102-110/embryos) and the media was collected for analysis from 4 different culture media and analyzed for PIF levels. Also, mouse embryos were cultured at 2-cell stage (N = 10-15/embryos) for 72 hours and the media was collected for PIF analysis from 7 different cultures. Results are mean values+/- SEM of results of independent cultures. Chemiluminescent ELISA. Background levels were substracted from each experiment. Data is expressed as ng/ml.