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Figure 6 | Reproductive Biology and Endocrinology

Figure 6

From: Structural and histological characterization of oviductal magnum and lectin-binding patterns in Gallus domesticus

Figure 6

Immunohistochemical analysis by laser confocal microscopy of the oviductal magnum from juvenile (10-week-old) and egg-laying adult (30-week-old) chickens. Oviductal magnum sections were immunostained with anti-OVA, anti-ESR1, and anti-PGR, and co-stained with lectin-WGA and DAPI. In the juvenile oviduct, anti-ESR1 and anti-PGR antibodies stained the epithelium slightly and rare staining occurred with the anti-OVA antibody. In the adult oviduct, the anti-OVA antibody stained the tubular gland layers located toward the outer layer (d, j), but not the luminal epithelium, which stained for lectin WGA only (g). The anti-ESR1 antibody strongly stained the tubular gland layers toward the outer layer (e, k) and luminal epithelium (h), but not the tubular gland in the inner layer (k). The anti-PGR antibody broadly stained the tubular gland (f, l) and epithelium (i). Arrows, luminal surface of the oviductal epithelium; Il, inner layer of the tubular gland; Ol, outer layer of the tubular gland. Bars = 100 μm (d-f) and 25 μm (a-c and g-l).

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